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pbabe puro tpr met  (Addgene inc)


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    Structured Review

    Addgene inc pbabe puro tpr met
    Pbabe Puro Tpr Met, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+puro+tpr+met/pBABE-puro+TPR-Met+(Plasmid+%2310902)/pm41923199-218-6-7
    Average 93 stars, based on 12 article reviews
    pbabe puro tpr met - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Receptor-type Protein Tyrosine Phosphatase β (RPTP-β) Directly Dephosphorylates and Regulates Hepatocyte Growth Factor Receptor (HGFR/Met) Function
    Article Snippet: .. Full-length human RPTP-β cDNA was subcloned into pShuttle mammalian expression vector (pShuttle RPTP-β, Clontech Laboratories, Inc., Palo Alto, CA). cDNA fragment encoding human TPR-Met fusion protein was generated by PCR from pBABE-puro TPR-Met (Addgene, Inc.) as a template using the following primers: forward, 5′-ACCATGGCGGCGGTGTTGCAGCA-3′; and reverse, 5′-TGATGTCTCCCAGAAGGAGG-3′. ..

    Article Title: Receptor-type Protein Tyrosine Phosphatase β (RPTP-β) Directly Dephosphorylates and Regulates Hepatocyte Growth Factor Receptor (HGFR/Met) Function
    Article Snippet: .. Plasmid Construction Full-length human RPTP-β cDNA was subcloned into pShuttle mammalian expression vector (pShuttle RPTP-β, Clontech Laboratories, Inc., Palo Alto, CA). cDNA fragment encoding human TPR-Met fusion protein was generated by PCR from pBABE-puro TPR-Met (Addgene, Inc.) as a template using the following primers: forward, 5′-ACCATGGCGGCGGTGTTGCAGCA-3′; and reverse, 5′-TGATGTCTCCCAGAAGGAGG-3′. ..

    Plasmid Preparation:

    Article Title: Receptor-type Protein Tyrosine Phosphatase β (RPTP-β) Directly Dephosphorylates and Regulates Hepatocyte Growth Factor Receptor (HGFR/Met) Function
    Article Snippet: .. Full-length human RPTP-β cDNA was subcloned into pShuttle mammalian expression vector (pShuttle RPTP-β, Clontech Laboratories, Inc., Palo Alto, CA). cDNA fragment encoding human TPR-Met fusion protein was generated by PCR from pBABE-puro TPR-Met (Addgene, Inc.) as a template using the following primers: forward, 5′-ACCATGGCGGCGGTGTTGCAGCA-3′; and reverse, 5′-TGATGTCTCCCAGAAGGAGG-3′. ..

    Article Title: Receptor-type Protein Tyrosine Phosphatase β (RPTP-β) Directly Dephosphorylates and Regulates Hepatocyte Growth Factor Receptor (HGFR/Met) Function
    Article Snippet: .. Plasmid Construction Full-length human RPTP-β cDNA was subcloned into pShuttle mammalian expression vector (pShuttle RPTP-β, Clontech Laboratories, Inc., Palo Alto, CA). cDNA fragment encoding human TPR-Met fusion protein was generated by PCR from pBABE-puro TPR-Met (Addgene, Inc.) as a template using the following primers: forward, 5′-ACCATGGCGGCGGTGTTGCAGCA-3′; and reverse, 5′-TGATGTCTCCCAGAAGGAGG-3′. ..

    Article Title: Aberrant MET activation impairs perinuclear actin cap organization with YAP1 cytosolic relocation
    Article Snippet: .. pTK93_Lifeact-mCherry plasmid was obtained from Prof. Yosef Yarden Laboratory, as used here while pBABE-puro TPR-Met was purchased from Addgene. ..

    Article Title: Aberrant MET activation impairs perinuclear actin cap organization with YAP1 cytosolic relocation.
    Article Snippet: .. COMMUNICATIONS BIOLOGY | (2023) 6:1044 | https://doi.org/10.1038/s42003-023-05411-y | www.nature.com/commsbio 13 Retroviral and lentiviral transduction. pTK93_Lifeact-mCherry plasmid was obtained from Prof. Yosef Yarden Laboratory, as used here37 while pBABE-puro TPR-Met was purchased from Addgene. ..

    Generated:

    Article Title: Receptor-type Protein Tyrosine Phosphatase β (RPTP-β) Directly Dephosphorylates and Regulates Hepatocyte Growth Factor Receptor (HGFR/Met) Function
    Article Snippet: .. Full-length human RPTP-β cDNA was subcloned into pShuttle mammalian expression vector (pShuttle RPTP-β, Clontech Laboratories, Inc., Palo Alto, CA). cDNA fragment encoding human TPR-Met fusion protein was generated by PCR from pBABE-puro TPR-Met (Addgene, Inc.) as a template using the following primers: forward, 5′-ACCATGGCGGCGGTGTTGCAGCA-3′; and reverse, 5′-TGATGTCTCCCAGAAGGAGG-3′. ..

    Article Title: Receptor-type Protein Tyrosine Phosphatase β (RPTP-β) Directly Dephosphorylates and Regulates Hepatocyte Growth Factor Receptor (HGFR/Met) Function
    Article Snippet: .. Plasmid Construction Full-length human RPTP-β cDNA was subcloned into pShuttle mammalian expression vector (pShuttle RPTP-β, Clontech Laboratories, Inc., Palo Alto, CA). cDNA fragment encoding human TPR-Met fusion protein was generated by PCR from pBABE-puro TPR-Met (Addgene, Inc.) as a template using the following primers: forward, 5′-ACCATGGCGGCGGTGTTGCAGCA-3′; and reverse, 5′-TGATGTCTCCCAGAAGGAGG-3′. ..

    Polymerase Chain Reaction:

    Article Title: Receptor-type Protein Tyrosine Phosphatase β (RPTP-β) Directly Dephosphorylates and Regulates Hepatocyte Growth Factor Receptor (HGFR/Met) Function
    Article Snippet: .. Full-length human RPTP-β cDNA was subcloned into pShuttle mammalian expression vector (pShuttle RPTP-β, Clontech Laboratories, Inc., Palo Alto, CA). cDNA fragment encoding human TPR-Met fusion protein was generated by PCR from pBABE-puro TPR-Met (Addgene, Inc.) as a template using the following primers: forward, 5′-ACCATGGCGGCGGTGTTGCAGCA-3′; and reverse, 5′-TGATGTCTCCCAGAAGGAGG-3′. ..

    Article Title: Modeling pediatric low-grade glioma heterogeneity using human forebrain organoids.
    Article Snippet: .. TPR-MET was amplified by PCR from pBABE-puro-TPR-MET (Addgene, 10902), p53 (R273C) was cloned from pCMV-Neo-Bam-p53R273C (a kind gift from Alberto Inga), while BRAF(V600E)-ires-Venus was cloned starting from pBabe-BRaf(V600E) (Addgene, 17544). ..

    Article Title: Receptor-type Protein Tyrosine Phosphatase β (RPTP-β) Directly Dephosphorylates and Regulates Hepatocyte Growth Factor Receptor (HGFR/Met) Function
    Article Snippet: .. Plasmid Construction Full-length human RPTP-β cDNA was subcloned into pShuttle mammalian expression vector (pShuttle RPTP-β, Clontech Laboratories, Inc., Palo Alto, CA). cDNA fragment encoding human TPR-Met fusion protein was generated by PCR from pBABE-puro TPR-Met (Addgene, Inc.) as a template using the following primers: forward, 5′-ACCATGGCGGCGGTGTTGCAGCA-3′; and reverse, 5′-TGATGTCTCCCAGAAGGAGG-3′. ..

    Article Title: A slow-cycling/quiescent cells subpopulation is involved in glioma invasiveness
    Article Snippet: .. TPR-MET was amplified by PCR from pBABE-puro-TPR-MET (Addgene, 10902). ..

    Amplification:

    Article Title: Modeling pediatric low-grade glioma heterogeneity using human forebrain organoids.
    Article Snippet: .. TPR-MET was amplified by PCR from pBABE-puro-TPR-MET (Addgene, 10902), p53 (R273C) was cloned from pCMV-Neo-Bam-p53R273C (a kind gift from Alberto Inga), while BRAF(V600E)-ires-Venus was cloned starting from pBabe-BRaf(V600E) (Addgene, 17544). ..

    Article Title: A slow-cycling/quiescent cells subpopulation is involved in glioma invasiveness
    Article Snippet: .. TPR-MET was amplified by PCR from pBABE-puro-TPR-MET (Addgene, 10902). ..

    Clone Assay:

    Article Title: Modeling pediatric low-grade glioma heterogeneity using human forebrain organoids.
    Article Snippet: .. TPR-MET was amplified by PCR from pBABE-puro-TPR-MET (Addgene, 10902), p53 (R273C) was cloned from pCMV-Neo-Bam-p53R273C (a kind gift from Alberto Inga), while BRAF(V600E)-ires-Venus was cloned starting from pBabe-BRaf(V600E) (Addgene, 17544). ..

    Retroviral:

    Article Title: Aberrant MET activation impairs perinuclear actin cap organization with YAP1 cytosolic relocation.
    Article Snippet: .. COMMUNICATIONS BIOLOGY | (2023) 6:1044 | https://doi.org/10.1038/s42003-023-05411-y | www.nature.com/commsbio 13 Retroviral and lentiviral transduction. pTK93_Lifeact-mCherry plasmid was obtained from Prof. Yosef Yarden Laboratory, as used here37 while pBABE-puro TPR-Met was purchased from Addgene. ..

    Transduction:

    Article Title: Aberrant MET activation impairs perinuclear actin cap organization with YAP1 cytosolic relocation.
    Article Snippet: .. COMMUNICATIONS BIOLOGY | (2023) 6:1044 | https://doi.org/10.1038/s42003-023-05411-y | www.nature.com/commsbio 13 Retroviral and lentiviral transduction. pTK93_Lifeact-mCherry plasmid was obtained from Prof. Yosef Yarden Laboratory, as used here37 while pBABE-puro TPR-Met was purchased from Addgene. ..



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